Companion App · SYN APSE

Conversor LIF

Convert Leica .lif microscopy files into per-channel TIFFs — ready for SYN APSE analysis.

Runs locally on your laboratory computer. Your data never leaves the machine.

Latest release · all versions

SYN APSE · Conversor LIF
What it does

From raw .lif to analysis-ready TIFFs in seconds

The Conversor LIF replaces the manual, error-prone Fiji/ImageJ flow that researchers normally use to extract per-channel TIFFs from Leica confocal acquisitions. It enumerates every series in a .lif file, computes a maximum intensity projection (or preserves the full Z-stack), and writes a clean Bio-Formats-style TIFF per series and channel — ready to be ingested by the SYN APSE analysis worker. A batch TIF → MIP mode also takes a folder of already-exported per-channel TIFF Z-stacks — organised by experiment and well — and projects each one to MIP with the same worker-ready naming, no .lif required.

Automatic discovery

Detects every series, channel, LUT and pixel size from the .lif header. No XML wrangling, no per-file configuration.

Per-channel TIFFs & MIP

Bio-Formats-compatible naming {lif} - Image001 - C=0.tif. ResolutionUnit and µm/px embedded so Fiji shows the correct scale. The batch TIF → MIP mode reuses the exact same naming for TIFFs you already exported.

Manifest per well

A _manifest.json captures SHA-256 of the source, projection mode, excluded channels and LUTs — full analytical traceability.

Why local

Your data stays on your machine

Microscopy files often contain unpublished, confidential or pre-clinical data. The Conversor LIF runs entirely on the laboratory computer — there is no upload, no cloud step, no telemetry. Compatible with institutional confidentiality clauses and non-disclosure agreements out of the box.

  • ✓ 100% local processing
  • ✓ No account required
  • ✓ No telemetry, no analytics
  • ✓ Auditable open-source codebase
Installation

A couple of steps on any operating system

Windows 10 / 11

  1. Download the .exe installer.
  2. Double-click; if SmartScreen warns, click More info → Run anyway.
  3. Follow the wizard. WebView2 is installed automatically if missing.
  4. Launch from the Start Menu shortcut.

macOS 11+ · Apple Silicon & Intel

  1. Pick the right .dmg above — Apple Silicon (M1/M2/M3/M4) or Intel (pre-2020 Macs).
  2. Open the .dmg, drag the app to Applications.
  3. First launch: right-click → Open → Open (Gatekeeper bypass).
  4. If macOS still blocks it: System Settings → Privacy & Security → Open Anyway.
  5. Launch from Launchpad on subsequent runs.

Linux · x86_64 & ARM64

  1. Easiest: download the .tar.gz, extract it, and run the app inside — no FUSE, no special permissions (the exact command is shown under the download button).
  2. AppImage: make it executable (chmod +x) and launch it. On newer Ubuntu, if it doesn't open, run it with --appimage-extract-and-run.
  3. Needs GTK + WebKit, preinstalled on most Linux desktops.
  4. Built for Ubuntu 22.04+ · x86_64 & ARM64 (incl. NVIDIA Jetson).
About the security warnings: these installers are not yet signed with an Apple Developer ID or a Windows Authenticode certificate, so both operating systems will surface a one-time warning the first time the app is launched. The signing infrastructure is on our roadmap; the application itself is safe, fully open-source, and identical across platforms.